ABSTRACT
OBJECTIVE: To study the chemical ethyl acetate-soluble constituents of the leaves of Callicarpa kwangtungensis Chun. METHODS: The compounds were isolated and purified by column chromatography using silica gel, Sephadex LH-20, ODS and MPLC. The structures of the compounds were identified according to their physicochemical properties and spectroscopic methods. RESULTS: Thirteen compounds were isolated and identified as 5,7,2’,6’-tetrahydroxyflavone(1), 2,3-dehydrosilychristin(2), silychristin A(3), dihydroquercetin(4), syringaresinol(5), medioresinol(6),(7R,8S)-3,5’-dimethoxy-4’,7-epoxy-8,3’-neolignane -5,9,9’-triol(7),(2S,3R)-methyl-7-hydroxy-2-(4-hydroxy-3-methoxyphenyl)-3-(hydroxymethyl)-2,3-dihydrobenzofuran-5-carboxylate(8), curcasinlignan B(9), vanillin(10), 19α-hydroxyursolic acid(11), 2α,3β,19α,23-tetrahydroxy-12-ene-28-oleanolic acid(12), and euscaphic acid(13). CONCLUSION: All these compounds were isolated from this plant for the first time.
ABSTRACT
Objective To study the ethyl acetate-soluble chemical constituents of Callicarpa kwangtungensis. Methods The chemical constituents were isolated by column chromatography on silica gel, ODS, Sephadex LH-20, and MPLC. Their chemical structures were elucidated on the basis of special analysis. Results Sixteen compounds were isolated and identified as carpeside B (1), loliolide (2), (3S,6E,10R)-10-β-D-glucopyranosyloxy-3,11-dihydroxy-3,7,11-trimethyldodeca-1,6-dine (3), lanceolatin A (4), daidzein (5), secroisolariciresinol (6), (7S,8R)-4,9,9'-trihydroxy-3,3'-dimethoxy-7,8-dihydrobenzofuran-1'-propylneolignan (7), silybin A (8), isosilybin A (9), isosilybin B (10), 2,3-dehydrosilyhin (11), silychristin A (12), silychristin B (13), isosilychristin (14), silydianin (15), and nigaichigoside F2 (16). Conclusion Compounds 4-15 are isolated from the genus Callicarpa for the first time.
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Objective: To establish HPLC fingerprints of Callicarpa kwangtungensis from different origins, which can be used in the evaluation for its quality control. Methods: The fingerprints of 15 batches of C. kwangtungensis were further evaluated by chemometrics methods. The similarity analyzed with "Similarity Evaluation System for Chromatographic Fingerprint of Chinese Materia Medica 2004A", and hierarchical clustering analysis (HCA) and principal component analysis (PCA) were performed by SPSS 19.0. Results: There were 12 common peaks, and the similarity degrees of 15 batches of samples were more than 0.644, and showed that all the samples from different origins were of good consistency. The samples were divided into four clusters by HCA. The result of PCA showed that the three factors were chosen, the quality of samples could be evaluated basically. According to the composite score, the quality of C. kwangtungensis from different origins, Hunan Huitong, Guangxi Guilin, Jiangxi Wuning, Jiangxi Nanchang, Jiangxi Pingxiang, and Jiangxi Yichun were better than others. Conclusion: The method is simple, reproducible, and reliable, than it can be used for quality control and evaluation of C. kwangtungensis from different origins.
ABSTRACT
@#Nine compounds were isolated from the aerial part of Callicarpa kwangtungensis chun by various column chromatographic methods. Their structures were identified as pinnatifidanoid A(1), blumenol C(2), megastigman-5- ene-3β, 9R-diol(3), 3β-hydroxyurs-12-en-28-oic acid(4), kaji-ichigoside F1(5), 1, 4-terephthalic acid(6), syringic acid(7), vanillic acid(8), and 3, 5-dimethoxy-4-methylbenzyl alcohol(9)on the basis of spectral analysis. C13 nor-isoprenoids of 1-3, and compounds 5, 6 and 9, were isolated from the genus Callicarpa for the first time.
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Objective: To study the technology for separation and purification of phenylethanoid glycosides from Callicarpa kwangtungensis by macroporous resin. Methods: The absorption and separation properties of 10 kinds of absorption resins were studied and the separation and purification technological process of phenylethanoid glycosides from C. kwangtungensis were optimized by HPLC with the total content of forsythiaside B and poliumoside as an index. Results: Using X-5 resin column as adsorbent, the optional conditions were as follows: loading qualities were 32.79 mg/g, the resin was washed by 4 BV distilled water to remove impurity and 7 BV 30% ethanol to elute phenylethanoid glycosides, the eluting velocity was 2 BV/h, and the content of phenylethanoid glycosides in the extract was 52.8% (n = 3). Conclusion: This technology is simple and feasible, and the process with X-5 resin is an effective method to separate and purify phenylethanoid glycosides from C. kwangtungensis.